Keywords: Mouse Mutants Production, quality controls, ES cells colture,Crispr/Cas9,  INFRAFRONTIER

Phd in Cell and Developmental Biology- Mouse mutant production for UE (EUCOMM, EUCOMM Tools, IMPC); “High-throughput” quality controls on  mutant murine strains and embryonic stem cells; genotype and phenotype characterization of mutant mice produced (EMMA/INFRAFRONTIER). Development and application of Crisp / Cas technique for the production of mutant murine strains. Culture, expansion and maintenance of mouse ES cells. Handling of animals and surgical techniques

Education:

• 2020- Phd in Cell and Developmental Biology – University “La Sapienza” of Rome – 32 A. Moro pl. Rome
• 2012- Master’s degree in Biology Applied to Biomedical Research– University “La Sapienza” of Rome – 32 A. Moro pl. Rome
• 2013- Enabling Biological Job- University “La Tuscia” in Viterbo

Positions

• 2021-Technologist   CNR Level   III, N.Matr. 21077
• 2014 – 2021 – Research Fellowship CNR-IBCN Campus Buzzati-Traverso – 32 Ramarini st. 00015 Monterotondo

 

1. Three-Dimensional X-ray Imaging of β-Galactosidase Reporter Activity by Micro-CT: Implication for Quantitative Analysis of Gene Expression.
Ermakova O, Orsini T, Fruscoloni P, Chiani F, Gambadoro A, Putti S, Cirilli M, Mezzi A, Kaciulis S, Pasquini M, Raspa M, Scavizzi F, Tocchini-Valentini GP.
Brain Sci. 2021 Jun 4;11(6):746. doi: 10.3390/brainsci11060746.
PMID: 34199780

2. Functional loss of Ccdc1 51 leads to hydrocephalus in a mouse model of primary ciliary dyskinesia.
Chiani F, Orsini T, Gambadoro A, Pasquini M, Putti S, Cirilli M, Ermakova O, Tocchini-Valentini GP.
Dis Model Mech. 2019 Aug 2;12(8). pii: dmm038489. doi: 10.1242/dmm.038489.

3. Identification of genes required for eye development by high-throughput screening of mouse knockouts.
Moore BA, Leonard BC, Sebbag L, Edwards SG, Cooper A, Imai DM, Straiton E, Santos L,Reilly C, Griffey SM, Bower L, Clary D, Mason J, Roux MJ, Meziane H, Herault Y; International Mouse Phenotyping Consortium, McKerlie C, Flenniken AM, Nutter LMJ, Berberovic Z, Owen C, Newbigging S, Adissu H, Eskandarian M, Hsu CW, Kalaga S, Udensi U, Asomugha C, Bohat R, Gallegos JJ, Seavitt JR, Heaney JD, Beaudet AL, Dickinson ME, Justice MJ, Philip V, Kumar V, Svenson KL, Braun RE, Wells S, Cater H, Stewart M, Clementson-Mobbs S, Joynson R, Gao X, Suzuki T, Wakana S, Smedley D, Seong JK, Tocchini-Valentini G, Moore M, Fletcher C, Karp N, Ramirez-Solis R, White JK, de Angelis MH, Wurst W, Thomasy SM, Flicek P, Parkinson H, Brown SDM, Meehan TF, Nishina PM, Murray SA, Krebs MP, Mallon AM, Lloyd KCK, Murphy CJ, Moshiri A.
Commun Biol. 2018 Dec 21;1:236. doi: 10.1038/s42003-018-0226-0. eCollection 2018. Erratum in: Commun Biol. 2019 Mar 7;2:97.

4.Identification of genetic elements in metabolism by high-throughput mouse phenotyping
Rozman J, Rathkolb B, Oestereicher MA, Schütt C, Ravindranath AC, Leuchtenberger S, Sharma S, Kistler M, Willershäuser M, Brommage R, Meehan TF, Mason J, Haselimashhadi H; IMPC Consortium, Hough T, Mallon AM, Wells S, Santos L, Lelliott CJ, White JK, Sorg T, Champy MF, Bower LR, Reynolds CL, Flenniken AM, Murray SA, Nutter LMJ, Svenson KL, West D, Tocchini-Valentini GP, Beaudet AL, Bosch F, Braun RB, Dobbie MS, Gao X, Herault Y, Moshiri A, Moore BA, Kent Lloyd KC, McKerlie C, Masuya H, Tanaka N, Flicek P, Parkinson HE, Sedlacek R, Seong JK, Wang CL, Moore M, Brown SD, Tschöp MH, Wurst W, Klingenspor M, Wolf E, Beckers J, Machicao F, Peter A, Staiger H, Häring HU, Grallert H, Campillos M, Maier H, Fuchs H, Gailus- Durner V, Werner T, Hrabe de Angelis M.
Nat Commun. 2018 Jan 18;9(1):288. doi: 10.1038/s41467-017-01995-2.

5.A large scale hearing loss screen reveals an extensive unexplored genetic landscape for auditory dysfunction
Bowl MR, Simon MM, Ingham NJ, Greenaway S, Santos L, Cater H, Taylor S, Mason J, Kurbatova N, Pearson S, Bower LR, Clary DA, Meziane H, Reilly P, Minowa O, Kelsey L; International Mouse Phenotyping Consortium, Tocchini- Valentini GP, Gao X, Bradley A, Skarnes WC, Moore M, Beaudet AL, Justice MJ, Seavitt J, Dickinson ME, Wurst W, de Angelis MH, Herault Y, Wakana S, Nutter LMJ, Flenniken AM, McKerlie C, Murray SA, Svenson KL, Braun RE, West DB, Lloyd KCK, Adams DJ, White J, Karp N, Flicek P, Smedley D, Meehan TF, Parkinson HE, Teboul LM, Wells S, Steel KP, Mallon AM, Brown SDM.
Nat Commun. (2017) Oct 12;8(1):886. doi: 10.1038/s41467-017- 00595-4

6.Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium
Meehan TF, Conte N, West DB, Jacobsen JO, Mason J, Warren J, Chen CK, Tudose I, Relac M, Matthews P, Karp N, Santos L, Fiegel T, Ring N, Westerberg H, Greenaway S, Sneddon D, Morgan H, Codner GF, Stewart ME, Brown J, Horner N; International Mouse Phenotyping Consortium, Haendel M, Washington N, Mungall CJ, Reynolds CL, Gallegos J, Gailus- Durner V, Sorg T, Pavlovic G, Bower LR, Moore M, Morse I, Gao X, Tocchini- Valentini GP, Obata Y, Cho SY, Seong JK, Seavitt J, Beaudet AL, Dickinson ME, Herault Y, Wurst W, de Angelis MH, Lloyd KCK, Flenniken AM, Nutter LMJ, Newbigging S, McKerlie C, Justice MJ, Murray SA, Svenson KL, Braun RE, White JK, Bradley A, Flicek P, Wells S, Skarnes WC, Adams DJ, Parkinson H, Mallon AM, Brown SDM, Smedley D. Nat Genet. (2017) Jun 26. doi: 10.1038/ng.3901

7.Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function.
Zorzi V, Paciello F, Ziraldo G, Peres C, Mazzarda F, Nardin C, Pasquini M, Chiani F, Raspa M, Scavizzi F, Carrer A, Crispino G, Ciubotaru CD, Monyer H, Fetoni AR, M Salvatore A, Mammano F.
Front Mol Neurosci. 2017 Nov 28;10:379. doi: 10.3389/fnmol.2017.00379. eCollection 2017. PMID: 29234270

8.High-throughput discovery of novel developmental phenotypes
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A; International Mouse Phenotyping Consortium; Jackson Laboratory; Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource Center, MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA.
Nature (2016) Sep 14;537(7621):508-514. doi:10.1038/nature19356.