Daniela Spano

Research Areas: Molecular oncology and Pathology

Napoli – Research Area NA1
daniela.spano@ibbc.cnr.it
Research FocusCurriculumKey Pubblications

Keywords: Molecular Oncology, Cellular and Molecular Biology, Biochemistry

The main interest of Dr. Spano is the molecular oncology. Her studies are focused on the unravelling the molecular basis of cancer development by dissecting the functional role of proteins/enzymes of interest in the regulation of signalling pathways and cellular processes involved in cancer development and progression. Her field is not only the basic research but also the translational research. In fact she performs in vitro functional studies in order to assess the anti-cancer properties of small molecules that are able to target the proteins/enzymes object of her studies.

Education:

  • February 2003: PhD in Cellular and Molecular Biology and Pathology XIV cycle at the University of Naples “Federico II”
  • February 1997: Degree with full marks and honours in Biology at Faculty of Mathematical, Physical and Natural Sciences of the University of Naples “Federico II”.

Positions: Researcher CNR

Honours/Awards:

  • 2014 – 2019: Postdoctoral Research Fellowship at the Institute of Protein Biochemistry – CNR, Naples, Italy.
  • 2012 – 2014: Postdoctoral Research Fellowship at the Institute of Biostructure and Bioimaging – CNR, Naples, Italy.
  • 2010 – 2012: Postdoctoral Research Fellowship at Department of Biochemistry and Medical Biotechnology of University of Naples “Federico II”, Naples, Italy.
  • 2004 – 2007 : Fellowship at CEINGE within the agreement between CEINGE and Ministero dell’Istruzione dell’Università e della Ricerca (MIUR), as an expert researcher.
  • 2004: fellowship funded by FIRC (Italian Fondation for Cancer Research)

 
Patents:

Patent title: PYRIMIDO[5,4-d]PYRIMIDINE OR PYRIMIDINE DERIVATIVES COMPOUNDS AND USES THEREOF IN THE TREATMENT OF CANCER

Patent code: PCT28627

Patentees: Zollo Massimo, Galeone Aldo, Virgilio Antonella, Spano Daniela, De Antonellis Pasqualino

Year: September 2012

  1. Maria Luisa Barretta, Daniela Spano, Chiara D’Ambrosio, Romina Ines Cervigni, Andrea Scaloni, Daniela Corda and Antonino Colanzi. Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2. Nat Commun. 2016 May 31;7:11727. ISSN: 2041-1723.

  2. Spano D, Marshall JC, Marino N, De Martino D, Romano A, Scoppettuolo MN, Bello AM, Di Dato V, Navas L, De Vita G, Medaglia C, Steeg PS, Zollo M. Dipyridamole prevents triple-negative breast-cancer progression. Clin Exp Metastasis. 2013 Jan;30(1):47-68. ISSN: 0262-0898

  3. Virgilio A, Spano D, Esposito V, Di Dato V, Citarella G, Marino N, Maffia V, De Martino D, De Antonellis P, Galeone A, Zollo M. Novel pyrimidopyrimidine derivatives for inhibition of cellular proliferation and motility induced by h-prune in breast cancer. Eur J Med Chem. 2012 Nov;57:41-50. ISSN: 0223-5234

  4. Spano D, Heck C, De Antonellis P, Christofori G, Zollo M. Molecular networks that regulate cancer metastasis. Semin Cancer Biol. 2012 Jun;22(3):234-49. ISSN: 1044-579X.

  5. Spano D, Russo R, Di Maso V, Rosso N, Terracciano LM, Roncalli M, Tornillo L, Capasso M, Tiribelli C, Iolascon A. Galectin-1 and its involvement in hepatocellular carcinoma aggressiveness. Mol Med. 2010 Mar;16(3-4):102-15. ISSN: 1076-1551.
  1. Varone A, Mariggiò S, Patheja M, Maione V, Varriale A, Vessichelli M, Spano D, Formiggini F, Lo Monte M, Brancati N, Frucci M, Del Vecchio P, D’Auria S, Flagiello A, Iannuzzi C, Luini A, Pucci P, Banci L, Valente C, Corda D. A signalling cascade involving receptor-activated phospholipase A2, glycerophosphoinositol 4-phosphate, Shp1 and Src in the activation of cell motility. Cell Commun Signal. 2019 Mar 1;17(1):20.

 

  1. Giuliana Catara, Giovanna Grimaldi, Laura Schembri, Daniela Spano, Gabriele Turacchio, Matteo Lo Monte, Andrea Rosario Beccari, Carmen Valente and Daniela Corda. PARP1-produced poly-ADP-ribose causes the PARP12 translocation to stress granules and impairment of Golgi complex functions. Sci Rep. 2017 Oct 25;7(1):14035.

 

  1. Maria Luisa Barretta, Daniela Spano, Chiara D’Ambrosio, Romina Ines Cervigni, Andrea Scaloni, Daniela Corda and Antonino Colanzi. Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2. Nat Commun. 2016 May 31;7:11727. ISSN: 2041-1723.

 

  1. Jiang WG, Sanders AJ, Katoh M, Ungefroren H, Gieseler F, Prince M, Thompson SK, Zollo M, Spano D, Dhawan P, Sliva D, Subbarayan PR, Sarkar M, Honoki K, Fujii H, Georgakilas AG, Amedei A, Niccolai E, Amin A, Ashraf SS, Ye L, Helferich WG, Yang X, Boosani CS, Guha G, Ciriolo MR, Aquilano K, Chen S, Azmi AS, Keith WN, Bilsland A, Bhakta D, Halicka D, Nowsheen S, Pantano F, Santini D. Tissue invasion and metastasis: Molecular, biological and clinical perspectives. Semin Cancer Biol. 2015 Dec;35 Suppl:S244-75. ISSN: 1044-579X.

 

  1. Cervigni RI, Bonavita R, Barretta ML, Spano D, Ayala I, Nakamura N, Corda D, Colanzi A. JNK2 controls fragmentation of the Golgi complex and G2/M transition trough phosphorylation of GRASP65. J Cell Sci. 2015 Jun 15;128(12):2249-60. ISSN: 0021-9533.

 

  1. De Antonellis P, Carotenuto M, Vandenbussche J, De Vita G, Ferrucci V, Medaglia C, Boffa I, Galiero A, Di Somma S, Magliulo D, Aiese N, Alonzi A, Spano D, Liguori L, Chiarolla C, Verrico A, Schulte JH, Mestdagh P, Vandesompele J, Gevaert K, Zollo M. Early targets of miR-34a in neuroblastoma. Mol Cell Proteomics. 2014 Aug;13(8):2114-31. ISSN: 1535-9476.

 

  1. Harrison LM, Muller SH, Spano D. Effects of the Ras Homolog Rhes on Akt/Protein Kinase B and Glycogen Synthase Kinase 3 Phosphorylation in Striatum. Neuroscience. 2013 Apr 16;236:21-30. ISSN: 0306-4522.

 

  1. Baiamonte BA, Lee FA, Brewer ST, Spano D, Lahoste GJ. Attenuation of Rhes Activity Significantly Delays the Appearance of Behavioral Symptoms in a Mouse Model of Huntington’s Disease. PLoS One. 2013;8(1):e53606. ISSN 1932-6203.

 

  1. Spano D, Marshall JC, Marino N, De Martino D, Romano A, Scoppettuolo MN, Bello AM, Di Dato V, Navas L, De Vita G, Medaglia C, Steeg PS, Zollo M. Dipyridamole prevents triple-negative breast-cancer progression. Clin Exp Metastasis. 2013 Jan;30(1):47-68. ISSN: 0262-0898.

 

  1. Virgilio A, Spano D, Esposito V, Di Dato V, Citarella G, Marino N, Maffia V, De Martino D, De Antonellis P, Galeone A, Zollo M. Novel pyrimidopyrimidine derivatives for inhibition of cellular proliferation and motility induced by h-prune in breast cancer. Eur J Med Chem. 2012 Nov;57:41-50. ISSN: 0223-5234

 

  1. Zollo M, Di Dato V, Spano D, De Martino D, Liguori L, Marino N, Vastolo V, Navas L, Garrone B, Mangano G, Biondi G, Guglielmotti A. Targeting monocyte chemotactic protein-1 synthesis with bindarit induces tumor regression in prostate and breast cancer animal models. Clin Exp Metastasis. 2012 Aug;29(6):585-601. ISSN: 0262-0898.

 

  1. Spano D, Zollo M. Tumor microenvironment: a main actor in the metastasis process. Clin Exp Metastasis. 2012 Apr;29(4):381-95. ISSN: 0262-0898.

 

  1. Spano D, Heck C, De Antonellis P, Christofori G, Zollo M. Molecular networks that regulate cancer metastasis. Semin Cancer Biol. 2012 Jun;22(3):234-49. ISSN: 1044-579X.

 

  1. Quintero GC, Spano D. Exploration of sex differences in Rhes effects in dopamine mediated behaviors. Neuropsychiatr Dis Treat. 2011;7:697-706. ISSN: 1176-6328.

 

  1. Lee FA, Baiamonte BA, Spano D, Lahoste GJ, Soignier RD, Harrison LM. Mice Lacking Rhes Show Altered Morphine Analgesia; Tolerance; and Dependence. Neurosci Lett. 2011 Feb 11;489(3):182-6. ISSN: 0304-3940.

 

  1. Matte A, Low PS, Turrini F, Bertoldi M, Campanella ME, Spano D, Pantaleo A, Siciliano A, De Franceschi L. Peroxiredoxin-2 (Prx2) expression is increased in beta thalassemic mouse red cells but displaced from the membrane as marker of oxidative stress. Free Radic Biol Med. 2010 Aug 1;49(3):457-66. ISSN: 0891-5849.

 

  1. Spano D, Russo R, Di Maso V, Rosso N, Terracciano LM, Roncalli M, Tornillo L, Capasso M, Tiribelli C, Iolascon A. Galectin-1 and its involvement in hepatocellular carcinoma aggressiveness. Mol Med. 2010 Mar;16(3-4):102-15. ISSN: 1076-1551.

 

  1. Persico M, Russo R, Persico E, Svelto M, Spano D, Andolfo I, La Mura V, Capasso M, Tiribelli C, Torella R, Iolascon A. SOCS3 and IRS-1 Gene Expression Differs Between Genotype 1 and Genotype 2 HCV-Infected HEPG2 Cells. Clin Chem Lab Med. 2009;47(10):1217-25. ISSN: 1434-6621.

 

  1. Schwarz K, Iolascon A, Verissimo F, Trede NS, Horsley W, Chen W, Paw BH, Hopfner KP, Holzmann K, Russo R, Esposito MR, Spano D, De Falco L, Heinrich K, Joggerst B, Rojewski MT, Perrotta S, Denecke J, Pannicke U, Delaunay J, Pepperkok R, Heimpel H. Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II. Nat Genet. 2009 Aug;41(8):936-40. ISSN: 1061-4036.

 

  1. Quintero GC, Spano D, Lahoste GJ, Harrison LM. The Ras homolog Rhes affects dopamine D1 and D2 receptor-mediated behavior in mice. Neuroreport. 2008 Oct 29;19(16):1563-6. ISSN: 0959-4965.

 

  1. Spano D, Cimmino F, Capasso M, D’Angelo F, Zambrano N, Terracciano L, Iolascon A. Changes of the Hepatic Proteome in Hepatitis B-Infected Mouse Model at Early Stages of Fibrosis. J Proteome Res. 2008 Jul 3;7(7):2642-2653. ISSN: 1535-3893.

 

  1. Errico F, Santini E, Migliarini S, Borgkvist A, Centonze D, Nasti V, Carta M, De Chiara V, Prosperetti C, Spano D, Herve D, Pasqualetti M, Di Lauro R, Fisone G, Usiello A. The GTP-binding protein Rhes modulates dopamine signalling in striatal medium spiny neurons. Mol Cell Neurosci. 2008 Feb;37(2):335-45. ISSN: 1044-7431.

 

  1. Persico M, Capasso M, Persico E, Svelto M, Russo R, Spano D, Crocè L, La Mura V, Moschella F, Masutti F, Torella R, Tiribelli C, Iolascon A. Suppressor of cytokine signaling 3 (SOCS3) expression and hepatitis C virus-related chronic hepatitis: Insulin resistance and response to antiviral therapy. Hepatology. 2007 Oct;46(4):1009-15. ISSN: 0270-9139.

 

  1. Cimmino F, Spano D, Capasso M, Zambrano N, Russo R, Zollo M, Iolascon A. Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line. J Proteome Res. 2007 Jul;6(7):2550-64. ISSN: 1535-3893.

 

  1. Persico M, Capasso M, Persico E, Masarone M, Renzo A, Spano D, Bruno S, Iolascon A. Interleukin-10 – 1082 GG polymorphism influences the occurrence and the clinical characteristics of hepatitis C virus infection. J Hepatol. 2006 Dec;45(6):779-85. ISSN: 0168-8278.

 

  1. Barone M, Spano D, Dapolito M, Centra M, Lasalandra C, Capasso M, Di Leo A, Volinia S, Arcelli D, Rosso N, Francavilla A, Tiribelli C, Iolascon A. Gene Expression Analysis in HBV Transgenic Mouse Liver: A Model to Study Early Events Related to Hepatocarcinogenesis. Mol Med. 2006 Apr-Jun;12(4-6):115-23. ISSN: 1076-1551.

 

  1. Barone M, Ladisa R, Di Leo A, Spano D, Francioso D, Aglio V, Amoruso A, Francavilla A, Iolascon A. Estrogen-Induced Proliferation in Cultured Hepatocytes Involves Cyclin D1, P21CIP1 and P27 KIP1 Dig Dis Sci. 2006 Mar;51(3):580-6. ISSN: 0163-2116.

 

  1. Spano D, Branchi I, Rosica A, Pirro MT, Riccio A, Mithbaokar P, Affuso A, Arra C, Campolongo P, Terracciano D, Macchia V, Bernal J, Alleva E, Di Lauro R. Rhes Is Involved in Striatal Function. Mol Cell Biol. 2004 Jul;24(13):5788-96. ISSN: 0270-7306.

 

 

Book Chapter

Daniela Spano and Massimo Zollo. Immune Cells Within the Tumor Microenvironment. Pages 1-23 M. Klink (ed.), Interaction of Immune and Cancer Cells, DOI 10.1007/978-3-7091-1300-4_1, © Springer-Verlag Wien 1 January 2014, Pages 1-263