Within Daniela Corda’s laboratory, my specific interest is to understand the physiological and pathological roles of mono-ADP-ribosylation, by investigating the enzymes and the substrates involved in this post-translational modification, along with their regulation.
We have started to characterise the localisation and function of intracellular enzymes catalysing this reaction (i.e. the PARPs) using antibody staining, RNAi and biochemical approaches for the identification of their physiological substrates. The data obtained so far demonstrated that some of these PARPs are involved in diverse cellular functions, ranging from the regulation of small-GTPases to the control of RNA as well as to the regulation of cell cycle progression, with particular emphasis on mitosis.
More specifically, by combining proteomics to advanced imaging techniques, we show an emergent role of mono-ADP-ribosylation in the regulation of intracellular membrane transport.
We are presently investigating this aspect, describing the pathways in which mono-ADP-ribosylation is involved and, if altered, how it can be connected to human diseases
February 2012: PhD in Life and Biomolecular Sciences. International Open University PhD. Project title: Identification and roles of intracellular substrates of mono-ADP-ribosylation.
July 2006: Masters in Medical Biotechnology, University of Florence, Italy Thesis: Role of the tyrosine kinase FAK in the repulsive response mediated by ephrinA1. (110/110, with honours).
September 2004: Degree in Biotechnology, University of Florence, Italy Thesis: Interaction between the Eph receptor and the low molecular weight tyrosine phosphatase LMW-PTP. Effects on the repulsive response. (110/110, with honours).
December 2019-present: Permanent Position as Researcher at the Institute of Biochemistry and Cell Biology (IBBC)- National Research Council (CNR, Naples).
June 2014-November 2019: fixed-term contract as Researcher at the Institute of Protein Biochemistry (IBP)- National Research Council (CNR, Naples).
January 2012-May 2014: post-doc position at the IBP-CNR (Naples).
January 2009-December 2011: fellowship from the Italian Federation for Cancer Research (FIRC, Milan, Italy).
April 2007-December 2008: doctoral Fellowship, Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Department of Cell Biology and Oncology.
September 2006- March 2007: co-co-co contract at the University of Florence, Department of clinical Physiology.
- 3-year Fellowship from the Italian Federation for Cancer Research (FIRC, Milan, Italy). Winners of Young Travel Grants (YTG):
- July 2009: YTG for the 34th FEBS Congress on Life’s Molecular Interaction, Prague.
- September 2009: EMBO FEBS YTG for a Summer School on “Proteins and their Networks – from specific to global analysis”. Spetsai.
- June 2010: YTG for the 10th Young Scientist Forum and 35th FEBS Congress on Molecules of Life, Gothenburg.
- PARPs and PAR as novel pharmacological targets for the treatment of stress granule-associated disorders. Grimaldi G, Catara G, Palazzo L, Corteggio A, Valente C, Corda D. Biochem Pharmacol. 2019 Sep;167: 64-75.
- ADP-ribosylation and intracellular traffic: An emerging role for PARP enzymes. Grimaldi G, Corda D. Biochem Soc Trans. 2019 Feb 28;47(1):357-370.
- In vitro techniques for ADP-ribosylated substrate identification. Grimaldi G, Catara G, Valente C, Corda D.Methods Mol Biol. 2018;1813:25-40.
- PARP1-produced poly-ADP-ribose causes the PARP12 translocation to stress granules and impairment of Golgi complex functions. Catara G*, Grimaldi G*, Schembri L, Spano D, Turacchio G, Lo Monte M, Beccari AR, Valente C, Corda D.Sci Rep. 2017 Oct 25;7(1):14035.
- Molecular mechanism and functional role of brefeldin A-mediated ADP-ribosylation of CtBP1/BARS. Colanzi A*, Grimaldi G*, Catara G, Valente C, Cericola C, Liberali P, Ronci M, Lalioti VS, Bruno A, Beccari AR, Urbani A, De Flora A, Nardini M, Bolognesi M, Luini A, Corda D.Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9794-9.